Phyto-Assisted Synthesis of Silver Nanoparticles using Solanum Nigrum and Antibacterial Activity Against Salmonella Typhi and Staphylococcus Aureus

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Venkat Kumar S., Karpagambigai S., Jacquline Rosy P., Rajeshkumar S. (2017). Phyto-Assisted Synthesis of Silver Nanoparticles using Solanum Nigrum and Antibacterial Activity Against Salmonella Typhi and Staphylococcus AureusMechanics, Materials Science & Engineering, Vol 9. doi:10.2412/mmse.86.22.967

Authors: Venkat Kumar S., Karpagambigai S., Jacquline Rosy P., Rajeshkumar S.

ABSTRACT. This study aims to provide a simple and eco-friendly approach for the synthesis of Ag NPs using medicinal plant Solanum nigrum aqueous leaf extract. Fresh leaf extract mediated the reduction of silver from higher excited state to the ground extract when mixed with 1 mM silver nitrate solution. Reduction led the synthesis of silver nanoparticle with the size range of 20-40 nm was confirmed by TEM analysis. UV-Vis analysis demonstrates the synthesis of Ag NPs by its standard peak due to SPR (Surface Plasmon Resonance). XRD analysis confirmed the crystalline nature of Ag NPs. It was further characterized by FT-IR for the confirmation of functional groups responsible for Ag NPs synthesis and elements analyzed using EDX. Our study also showed the high antibacterial effect of nanoparticle against disease causing virulent bacterial strains (Salmonella typhi and Staphylococcus aureus).

Keywords: silver nanoparticles, green synthesis, antibacterial activity, TEM, salmonella typhi

DOI 10.2412/mmse.86.22.967

References

[1] Kavishankar G.B, Lakshmidevi N, MahadevaMS.Phytochemicalanalusis and antimicrobial properties ofselected medicinal plants against bacteria associated with diabetic patients. International Journal of Pharma and BioSciences. 2 (4): 2011;509-518

[2] Ali NS, Singh K, Khan MI, Rani S. Protective effect of ethanolic extracts of Solanum nigrum on the blood sugar of albino rats. IJPSR. 1 (9): 2010; 97-99.

[3] Bhavani, G. Geetha, J. Santhoshkumar and S Rajeshkumar, (2015) Evaluation of Antibacterial action and Hepatoprotective efficiency of Solanum nigrum leaves extract on acetaminophen induced hepatotoxicity. Research J. Pharm. and Tech. 8 (7) : 893-900.

[4] Patels S, Gheewala N, Suthar A,, Shah A. 0In-vitrocytotoxicity activity of Solanumnigrum extracts againstHela cell lines and Vero cell lines. International journal ofpharmacy and pharmaceutical sciences. 1 (1): 2009;38-46.

[5] Sridhar TM, Josthna P, Naidu CV. Antifungal activity, phytochemical analysis of Solanumnigrum (L.)-animportant antiulcer medicinal plant. Journal ofEcobiotechnology 3 (7): 2011 (Linn.) – An important antiulcer medicinal plant. Journal ofExperimental Sciences. 2 (8): 2011; 24-29.

[6] Javed T, UsmanAA, Sana R, Sidra R, Sheikh R. In-vitroantiviral activity of Solanumnigrumagainst Hepatitis CVirus. Virology Journal.8:2011; 26.

[7] Jainu M, Devi CSS.Antioxidant effect of methanolicextractsofSolanumnigrumberries on aspirin induced gastricmucosalinjury.Indian Journal of Clinical Biochemistry, 19 (1): 2004; 57-61.

[8] Elhag RAM, Badwi MAE, Bakhiet AO, Galal M. Hepatoprotective activity of Solanumnigrumextracts onchemically induced liver damage in rats. Journal on Veterinary Medicine and Animal Health. 3 (4): 2011;45-50.

[9] Ravi V, Saleem TSM, Patel SS, Raamamurthy J Gauthaman K. Anti-inflammatory effect of methanolic extract of Solanumnigrum Linn berries. International Journal of Applied Research in Natural Products. 2 (2): 2009;33-36.

[10] Noel NW, Joseph AA, Helen OK, Steven SG, Asa A. Antiseizure activity of the aqueous leaf extract of Solanum nigrumlinn (solanaceae) in experimental animals.AfricanHealth Sciences.2008; 8 (2), 74-79.

[11] Akubugwo I. E, Obasi N.A, Chinyere G.C and Ugbogu A. Mineral and phytochemical contents in leaves of Amaranthus hybridus L and Solanum nigrum L. subjected todifferent processing methods. African Journal ofBiochemistry Research. 2 (2): 2008; 040-044.

[12] Rawani A, Ghosh A Chandra G.nMosquitolarvicidalactivities of Solanumnigrum leaf extract against Culexquinquefasciatus. Parasitol Res. 107: 2010;1235–1240.

[13] Balaji R, Prakash G, Suganyadevi P, Aravinthan K M.Evaluation of cardio protective Activity of Methanolic Extract Of Solanum nigrum Linn. in Rats. International Journal of Drug Development & Research. 3 (3):2011; 139-147.

[14] S Rajeshkumar (2016) Synthesis of silver nanoparticles using Fresh bark of Pongamia pinnata and its antibacterial action against gram positive and gram negative pathogen Resource-Efficient Technologies 2 (2016) 30–35.

[15] Jain, H.K.Daima, S.Kachhwaha, S.L.Kothari. Synthesis of plant-mediated silver nanoparticles using papaya fruit extract and evaluation of their anti-microbial activities. Digest Journal of Nanomaterials and Biostructures.2009; 4: 557–563.

[16] Kannan Badri Narayanan, Hyun Ho Park, NatarajanSakthivel Extracellular synthesis of mycogenic silver nanoparticles by Cylindrocladiumfloridanum and its homogeneous catalytic degradation of 4-nitrophenol SpectrochimicaActa Part A: Molecular and Biomolecular Spectroscopy 116 (2013) 485–490.

[17] Nakkala JR, Mata R, Kumar Gupta A, Rani Sadras S. Biological activities of green silver nanoparticles synthesized with Acorous calamus rhizome extract. Eur J Med Chem 2014;85:784–94.

[18] Kanagavalli, A. Mohamed Sadiq, Sathishkumar, S. Rajeshkumar. Plant Assisted Synthesis of Silver Nanoparticles Using Boerhaavia diffusa Leaves Extract and Evolution of Antibacterial Activity. Research J. Pharm. and Tech 2016; 9 (8):1064-1068.

[19] Ulug B, HalukTurkdemir M, Cicek A, Mete A. Role of irradiation in the green synthesis of silver nanoparticles mediated by Fig. (Ficus carica) leaf extract. Spectrochim Part A: Mol Biomol Spectrosc 2015;135:153–61.

[20] Geetha N, Geetha TS, Manonmani P, Thiyagarajan M. Green synthesis of silver nanoparticles using Cymbopogan Citratus (Dc) Stapf. Extract and its antibacterial activity. Aus J Basic Appl Sci 2014;8 (3):324–31.

[21] Masurkar SA, Chaudhari PR, Shidore VB, Kamble SP. Rapid biosynthesis of silver nanoparticles using Cymbopogan Citratus (Lemongrass) and its antimicrobial activity. Nano-Micro Lett 2011;3 (3):189–94.

[22] Kumarasamyraja D, jeganathan NS. Green synthesis of silver nanoparticles using aqueous extract of acalypha indica and its antimicrobial activity. Int J Pharm Biol Sci 2013;4 (3): 469–76.

[23] Kumar S, Daimary RM, Swargiary M, Brahma A, Kumar S, Singh M. Biosynthesis of silver nanoparticles using Premna herbacea leaf extract and evaluation of its antimicrobial activity against bacteria causing dysentery. Int J Pharm Biol Sci 2013;4 (4):378–84.

[24] Sadeghi B, Rostami A, Momeni SS. Facile green synthesis of silver nanoparticles using seed aqueous extract of Pistacia atlantica and its antibacterial activity. Spectrochim Part A: Mol Biomol Spectrosc 2015;134:326–32.

[25] Vijayaraghavan K, Nalini S, Prakash NU, Madhankumar D. One step green synthesis of silvernano/microparticles using extracts of Trachyspermum ammi and Papaver somniferum. Colloid Surf B Biointerfaces 2012;94:114–7.

[26] Thombre R, Parekh F, Patil N. Green synthesis of silver nanoparticles using seed extract of Argyreia nervosa. Int J Pharm Biol Sci 2014;5 (1):114–9.

[27] Sunita D, Tambhale D, Parag V, Adhyapak A. Facile green synthesis of silver nanoparticles using Psoralea corylifolia. Seed extract and their in-vitro antimicrobial activities. Int J Pharm Biol Sci 2014;5 (1):457–67.

[28] Gnanajobitha G., Rajeshkumar, S., Kannan C and Annadurai, G. 2012. Preparation and characterization of fruit-mediated silver nanoparticles using pomegranate extract and assessment of its antimicrobial activity (2013) Journal of Environmental nanotechnology 2 (1), 04-10.

[29] G Gnanajobitha, K Paulkumar, M Vanaja, S Rajeshkumar, C Malarkodi, G Annadurai and C Kannan Fruit mediated Synthesis of Silver Nanoparticles using Vitis vinifera and Evaluation of their Antimicrobial Efficacy (2013) Journal of Nanostructures in Chemistry 3 (67): 1-6.

[30] Rajeshkumar Phytochemical constituents of fucoidan (Padina tetrastromatica) and its assisted silver nanoparticles for enhanced antibacterial activity (2016) IET Nanobiotechnology doi: 10.1049/iet-nbt.2016.0099.

[31] Rajeshkumar S, Malarkodi C, Vanaja M,, Annadurai G Anticancer and enhanced antimicrobial activity of biosynthesizd silver nanoparticles against clinical pathogens Journal of Molecular Structure 1116 (2016) 165-173.

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